[4K UHD]
- Main audio: Dolby Atmos TrueHD: English, Dolby Digital 5.1: French, German, Italian, Spanish, Thai, 2.0: English ADS (UK)
- Main subtitles: Korean, English SDH, Chinese, Danish, Dutch, Finnish, German SDH, Italian SDH, Spanish, Norwegian, French, Swedish, Thai

[2D BD]
- Main audio: Dolby TrueHD: English, Dolby Digital 5.1: English, Portuguese, Czech, Hungarian, Polish, Russian, Thai, Turkish
- Main subtitles: Korean, English SDH, Arabic, Bahasa-Indonesian, Bulgarian, Chinese, Croatian, Czech, Estonian, Greek, Hebrew, Hungarian, Icelandic, Latvian, Lithuanian, Polish, Romanian, Serbian, Slovenian, Thai, Portuguese, Turkish, Ukrainian

[2D BD]
- BEHIND THE STORY: Frank Miller Tapes [1080p] (14:33)
- BTS:Preparing for Battle: Original Test [1080p] (6:39)
- BTS: The 300 - Fact or Fiction? [1080p] (24:33)
- BEHIND THE STORY: Webisode : Wardrobe (3:37)
- BEHIND THE STORY: Webisode: Lena Headey (1:43)
- BEHIND THE STORY: Webisode: Gerard Butler (4:04)
- BTS: Webisode: Fantastic Characters of 300(3:04)
- BEHIND THE STORY: Webisode: Scene Studies from 300 (3:13)
- BTS: Webisode: A Glimpse from the Set: Making 300 (3:24)
- BTS: Webisode: Culture of the Sparta City / State (2:11)
- BEHIND THE STORY: Webisode: Training the Actors (2:32)
- BEHIND THE STORY: Webisode: Rodrigo Santoro (2:26)
- BEHIND THE STORY:Making of 300 (Ver 2-BC 0361414) (5:49)
- Deleted Scenes w/Intro by Zack Snyder [1080p] (3:21)
- BTS: Webisode: Adapting the Graphic Novel (3:44)
- BEHIND THE STORY: Webisode: Stunt Work (4:09)
- BEHIND THE STORY: Webisode: Production Design (3:51)
- Who Were the Spartans? The Warriors of 300[1080p] (4:24)
- BEHIND THE STORY: Making 300 in Images (3:39)
- BTS:Audio Commentary by Director Zack Snyder, et al (116:21)

* Special Feature Audio: English
* Special feature subtitles: Korean, English SDH

In the Battle of Thermopylae of 480 BC an alliance of Greek city-states fought the invading Persian army in the mountain pass of Thermopylae. Vastly outnumbered, the Greeks held back the enemy in one of the most famous last stands of history. Persian King Xerxes lead a Army of well over 100,000 (Persian king Xerxes before war has about 170,000 army) men to Greece and was confronted by 300 Spartans, 700 Thespians and other Slave soldiers. Xerxes waited for 10 days for King Leonidas to surrender or withdraw left with no options he moved. The battle lasted for about 3 days and after which all 300 Spartans were killed. The Spartan defeat was not the one expected, as a local shepherd, named Ephialtes, defected to the Persians and informed Xerxes of a separate path through Thermopylae, which the Persians could use to outflank the Greeks.